The synthesis and utilization of cholesterol must be tightly regulated in order to prevent over-accumulation and abnormal deposition within the body. Regulation of cholesterol synthesis. Biosynthesis of cholesterol is directly regulated by the cholesterol levels present, though the homeostatic mechanisms. In mammals, intracellular levels of cholesterol and fatty acids are controlled through a feedback regulatory system mediated by a family of transcription factors. The Mevalonate pathway. This is the rate limiting step in cholesterol synthesis. Polytechnic Institute page on cholesterol synthesis (including regulation) Regulation of cholesterol synthesis. HMG-CoA Reductase, the rate-determining step on the pathway for synthesis of cholesterol, is a major control point. regulation of cholesterol biosynthesis. Obviously there are more questions than. regulation of cholesterol synthesis and 3-hydroxy-3-methylglutaryl coenzyme A Transcriptional regulation of cholesterol synthesis starts in the ER; When cholesterol is low, SREBP is sorted to the Golgi apparatus; Proteolytic cleavage in the. The amount of cholesterol that is synthesized in the liver is tightly regulated by dietary cholesterol levels. When dietary intake of cholesterol is high, synthesis. Cholesterol synthesis- Details of steps, regulation,transport of cholesterol, variations of serum cholesterol levels, hypolipidemic drugs HMG CoA reductase produces mevalonate, an important intermediate in the synthesis of cholesterol and essential nonsterol isoprenoids. The reductase is subject to an.