Protocol for cDNA synthesis and qRT-PCR cDNA Synthesis Superscript III 1st Strand Synthesis Kit. (Melting point step to check for primer dimer formation). I have RNA and performed cDNA synthesis. I want to check if there is cDNA or not. I checked OD260 before and after cDNA synthesis, it was 0.4 before reverse. SuperScript® Double-Stranded cDNA Synthesis Kit (200 cpm/pmol dCTP) × (3,030 pmol dNTP/μg cDNA) Catalog no. 11917-010 Size: 10 reactions Hi there, first of all just before a cDNA synthesis you should check the quality of the isolated RNA and decide what method (in fact primer/primers) of cDNA synthesis. All-in-One™ First-Strand cDNA Synthesis Kit For reliable first-strand cDNA synthesis from all RNA sources Cat. No. AORT-0020 (20 synthesis reactions) The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. cDNA Synthesis Kits at Biocompare.com. Compare specifications, prices, and reviews from leading suppliers. Use Biocomp Molecular Genetics II - Genetic Engineering Course (Supplementary notes) Figures showing examples of cDNA synthesis (currently 11 figures) Problem: Suggestion: Low Yield of cDNA: Check the integrity of the RNA by denaturing agarose gel electrophoresis (2). RNA should have a minimum A 260 /A. cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2). How to check if reverse transcription went OK and there is cDNA - (reply: 5).