Protocols; cDNA Synthesis Selection Chart; Protocols for cDNA Synthesis & RT-PCR Batch Method Protocol (S1560) cDNA synthesis in oligo (dT)25 magnetic beads (S1419) In genetics, complementary DNA (cDNA) is double-stranded DNA synthesized from a messenger RNA template in a reaction catalysed by the enzyme reverse transcriptase. Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical for obtaining high yields of quality, full-length cDNA that accurately represents the. Molecular Genetics II - Genetic Engineering Course (Supplementary notes) Figures showing examples of cDNA synthesis (currently 11 figures) cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. Reverse transcriptases (RTs) use an RNA template. qScript™ cDNA Synthesis. qScript™ cDNA Synthesis sets a new standard for reproducibility, specificity, speed and convenience in qPCR and RT-PCR applications. SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. cDNA is a more convenient way to work with the coding sequence than mRNA because RNA is very easily degraded by omnipresent RNases. This the main reason cDNA is. Our selection of kits for efficient and reproducible first strand cDNA synthesis includes either RevertAid or Maxima Reverse Transcriptases (RT). 2 Overview 2 2.1 The cDNA Synthesis System The cDNA Synthesis System provides the materials needed to rapidly and reliably synthesize double-stranded cDNA from mRNA.